Holland Lab | Publications

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Publications

2025

Chalek, K., Holland, G.P. “Prey-wrapping Spider Silk as a Model for Tough and Adaptable Protein Materials” Commun. Mater. 2026, 7, 24.

https://www.nature.com/articles/s43246-025-01039-z

Johnson, H.R., Chalek, K., Elathram, N., Chau, A.T., Domingo, A.R., Aldana, J.E., Nguyen, H., de Loera A., Duarte, B.A., Shapakidze, L., Onofrei, D., Debelouchina. G.T., Lorenz, C.D., Holland, G.P. “Arg–Tyr Cation–π Interactions Drive Phase Separation and β-sheet Assembly in Native Spider Dragline Silk” Proc. Natl. Acad. Sci. U.S.A. 2025, 122, e2523198122.

https://www.pnas.org/doi/abs/10.1073/pnas.2523198122

Forman, C.J., Onofrei, D., Stengel, D., Aldana, J.E., Paolini, C., Gianneschi, N.C., Holland, G.P. “Sequence-encoded Tubular Architectures in Disordered Spider Silk Proteins Revealed by Multiscale Simulations and NMR” PNAS Nexus 2025, 4, pgaf378.

https://academic.oup.com/pnasnexus/article/4/12/pgaf378/8363280

Johnson, H.R., Dhaliwal, H., Makasarashvili, N., Malki, J., Vasquez, F., Villareal, D., Garmann, R.F., Holland, G.P. “Distinct Dimerization Mechanisms in Silkworm and Spider Silk Proteins Revealed by Mass Photometry” J. Phys. Chem. Lett. 2025, 16, 11451-11457.

https://pubs.acs.org/doi/full/10.1021/acs.jpclett.5c02335

Li, Y., Duarte, B., Holland, G.P. “Calcium Environments, Proton Defects, and Facet-dependent Fluoride Interactions in Hydroxyapatite Nanostructures Probed by Multinuclear Solid-state NMR” Phys. Chem. Chem. Phys. 2025, 27, 23105-23115.

https://pubs.rsc.org/en/content/articlehtml/2025/cp/d5cp03614c

Peddireddy, K.R., Johnson, H.R., Holland, G.P., Robertson-Anderson, R.M. “Local Viscoelasticity of Denaturing Spider Silk Dope is Governed by Dynamic Hierarchical Intermolecular Interactions” Acta Biomaterilia. 2025, 205, 467-477.

https://www.sciencedirect.com/science/article/pii/S1742706125006737

Ezzatpour et al. “Broad-spectrum Synthetic Carbohydrate Receptors (SCRs) Inhibit Viral Entry Across Multiple Virus Families” Sci. Adv. 2025, 11, eady3554.

https://www.science.org/doi/10.1126/sciadv.ady3554

ZeuBel, L., Bargel, H. L., Holland, G.P., Scheibel, T. “Liquid-liquid Phase Separation of Spider Silk Proteins” Polymer J. 2025, 57, 831-843.

https://www.nature.com/articles/s41428-025-01039-3

Johnson, H.R., Foster, L., Domino, R.A., Holland, G.P., “Negative Stain TEM Imaging of Native Spider Silk Protein Superstructures” Ultramicroscopy 2025, 271, 114124. https://www.sciencedirect.com/science/article/pii/S0304399125000233

Chalek, K., Onofrei, D., Aldana, J.E., Johnson, H.R., Stengel, D., Chau, A.T., Domingo, A.R., Alcala, E., Tsui, E., Botello, F., Soni, A., Addison, B., Holland, G.P., “Unraveling the Molecular Origin of Prey-wrapping Spider Silk’s Unique Mechanical Properties and Assembly Process with NMR” Adv. Funct. Mater. 2025, 35, 2409321. https://onlinelibrary.wiley.com/doi/10.1002/adfm.202409321

2024

Li, Y., Lorenz, C.D., Holland, G.P., “Aspartic Acid Binding on Hydroxyapatite Nanoparticles with Varying Morphologies Investigated by Solid-state NMR Spectroscopy and Molecular Dynamics Simulation” Langmuir 2024, 40, 22824-22834.

https://pubs.acs.org/doi/10.1021/acs.langmuir.4c02880

Chalek, K., Soni, A., Lorenz, C.D., Holland, G.P., “Proline-Tyrosine Ring Interactions in Black Widow Dragline Silk Revealed by Solid-State Nuclear Magnetic Resonance and Molecular Dynamics Simulation” Biomacromolecules 2024, 25, 1916-1922.

https://doi.org/10.1021/acs.biomac.3c01351

Rachkov, A.G., Chalek, K., Yin, H., Xu, M., Schimpf, A.M.,Holland, G.P., “Redox Chemistries for Vacancy Modulation in Plasmonic Copper Phosphide Nanocrystals” ACS Nano 2024, 18, 5282-5296. https://doi.org/10.1021/acsnano.3c08962

2023

Shaidani, S., Jacobus, C., Sahoo, J.K., Harrington, K., Johnson, H. R., Foster, O., Cui, S., Hasturk, O., Falcucci, T, Chen, Y., Fletcher, M., Brooks, C., Holland, G.P., Kaplan, D.K.. “Silk Nanoparticle Synthesis: Tuning Size, Dispersity and Surface Chemistry for Drug Delivery” ACS Applied Nano Materials 2023, 10,18967-18977.https://doi.org/10.1021/acsanm.3c03451

Stengel, D., Saric, M., Johnson, H. R., Schiller, T., Diehl, J., Scheibel, T. Holland, G.P. “Tyrosine’s Unique Role in Hierarchical Assembly of Recombinant Spider Silk Proteins: From Spinning Dope to Fibers” Biomacromolecules 2023, 24,1463-1474.https://doi.org/10.1021/acs.biomac.2c01467

Stengel, D., Thai, R., Li, Y., Peters, N. M., Holland, G.P. “Biphasic Nature of Lipid Bilayers on Silica Nanoparticles and Evidence for an Interdigitated Phase” Soft Matter 2023, 19,1882-1889.https://doi.org/10.1039/D2SM01517J

2022

Li, Y., Deguzman M. A., Heise, J. E., Holland, G.P. “Synthesis and Structural Characterization of Morphologically Distinct Nanoscale Hydroxyapatites” J. Phys. Chem. C. 2022, 126, 12790-12801.https://pubs.acs.org/doi/full/10.1021/acs.jpcc.2c02383

Swanson, H. L., Couri, E. A., Sabando, C. J. P., Holland, G.P. “Assembly and Thermal-Induced Polymerization of Histidine on Fumed Silica Surfaces” ACS Earth Space Chem. 2022, 6, 1552-1562. https://doi.org/10.1021/acsearthspacechem.2c00059

2021

Stengel, D., Addison, J.B., Onofrei, D., Huynh, N.U., Youssef, G., Holland, G.P. “Hydration-Induced β-sheet Crosslinking of α-helical-rich Spider Prey-Wrapping Silk” Adv. Funct. Mater. 2021, 31, 2007161. IF = 16.836

Onofrei, D., Stengel, D., Jia, D., Johnson, H.R., Trescott, S., Soni, A., Addison, B., Muthukumar, M., Holland, G.P. “Investigating the Atomic and Mesoscale Interactions that Facilitate Spider Silk Protein Pre-assembly” Biomacromolecules 2021, 22, 3377-3385. IF = 6.988

Swanson, H.L., Pokhrel, S., Davidowski, S.K., Mädler, L., Brinker, C.J., Holland, G.P. “The Impact of Metal Doping on Fumed Silica Structure and Amino Acid Thermal Condensation Catalytic Properties” J. Mater. Sci. 2021, 56, 16916-16927. IF = 4.22

2020

Swanson, H.L., Guo, C., Cao, M., Addison J.B. and Holland, G.P.“Probing the Binding Modes and Dynamics of Histidine on Fumed Silica Surfaces by Solid-state NMR” Phys. Chem .Chem. Phys. 2020, 22, 20349-20361. IF = 3.567

Addison, J.B., Stengel, D., Bharadwaj, V.S., Happs, R.M., Doeppke, C., , Wang, T., Bomble, Y.J., Holland, G.P., Harman-Ware, A.E. “Selective 1D ¹³C-¹³C Spin-Diffusion Solid-State NMR Methods to Probe Spatial Arrangements in Biopolymers” J. Phys. Chem. B. 2020, 124, 9870-9083. IF = 2.857

2019

McGill, M., Holland, G.P., Kaplan, D.L. “Experimental Methods for Characterizing the Secondary Structure and Thermal Properties of Silk Proteins” Macromol. Rapid Commun. 2019, 40, 1800390. IF = 4.265

Kim, Y.H., Leriche, G., Diraviyam, K., Koyanagi, T., Gao, K., Onofrei, D., Patterson, J., Guha, A., Gianneschi, N., Holland, G.P., Gilson, M.K., Mayer, M., Sept, D., Yang, J. “Entropic Effects Enable Life at Extreme Temperatures” Science Advances 2019, 5, eaaw4783. IF = 12.804

Leriche, G., Onofrei, D., Stengel, D. Koyanagi, T., Holland, G.P., Yang, J. “Fusion of Bipolar Tetraether Lipid Membranes Without Enhanced Leakage of Small Molecules” Sci. Rep. 2019, 9, 1-11. IF = 4.122

Irazema, J.I., Stengel, D., Garcia, C.A., Addison, J.B., Samaan, G.N., Holland, G.P., Purse, B.W. “²H NMR Reveals Liquid State-like Dynamics of Arene Guests Inside Hexameric Pyrogallo[4]arene Capsules in the Solid State” Organic Chemistry Frontiers 2019, 6, 1361-1366. IF = 4.693

Polido, G., Xhi, X., Xu, D., Guo, C., Thai, R., Patterson, J.P., Gianneschi, N.C., Suchyna, T.M., Sachs, F., Holland, G.P. “Investigating the Interaction of Grammostola rosea Venom Peptides and Model Lipid Bilayers with Solid-state NMR and Electron Microscopy Techniques” Biochim. Biophys. Acta 2019, 1861, 151-160.* IF = 3.687

Parent, L.R., Onofrei, D., Xu, D., Stengel, D., Roehling, J.D., Addison, J.B., Forman, C. Amin, S.A, Cherry, B.R., Yarger, J.L., Gianneschi, N.C., Holland, G.P. “Hierarchical Spidroin Micellar Nanoparticles as the Precursors of Spider Silks” Microsc. Microanal. 2019, 25, 1346-1347.* IF = 2.673

2018

Addison, B., Onofrei, D., Stengel, D., Blass, B., Brenneman, B., Ayon, J., Holland, G.P. “Spider Prey Wrapping Silk is an α-helical Coiled-coil/β-sheet Hybrid Nanofiber” Chem. Commun. 2018, 54, 10746-10749. IF = 6.290

Parent, L.R., Onofrei, D., Xu, D., Stengel, D., Roehling, J.D., Addison, J.B., Forman, C. Amin, S.A, Cherry, B.R., Yarger, J.L., Gianneschi, N.C., Holland, G.P. “Hierarchical Spidroin Micellar Nanoparticles as the Fundamental Precursors of Spider Silks” Proc. Natl. Acad. Sci. U.S.A. 2018, 115, 11507-11512. IF = 9.661; Highlighted in The Washington Post, Newsweek, and C&E News

2017

Journey, S.N., Teppang, K.L., Garcia, C.A., Brim, S.A., Onofrei, D., Addison, J.B., Holland, G.P., Purse, B.W. “Mechanically Induced Pyrogallol [4] arene Hexamer Assembly in the Solid State Extends the Scope of Molecular Encapsulation” Chemical Science. 2017, 8, 7737–7745. IF = 8.668

Koyanagi, G., Cifelli, J.L., Leriche, G., Yep, Onofrei, D., Holland, G.P., Yang, J. “Thiol-triggered Release of Intraliposomal Content from Liposomes made of Extremophile-inspired Tetraether Lipids” Bioconjugate Chem. 2017, 28, 2041-2045. IF = 4.818

Guo, C., Jordan, J.S., Yarger, J.L., Holland, G.P. “Highly Efficient Fumed Silica Nanoparticles for Peptide Bond Formation: Converting Alanine to Alanine Anhydride” ACS Appl. Mater. Interfaces 2017, 9, 17653-17661.* IF = 7.504

Koyanagi, G., Cao, K.J., T., Leriche, Yep, Onofrei, D., Holland, G.P., Mayer, M., Sept, D., Yang, J. “Lipids Inspired by Extremophiles and Eukaryotes Afford Serum-stable Membranes with Low Leakage” Chemistry-A European Journal. 2017, 23, 6757-6762. IF = 5.771

2016

Gray, G.M., van der Vaart, A., Guo, C., Jones, J., Onofrei, D., Cherry, B.R., Yarger, J.L., Holland, G.P. “Secondary Structure Adopted by the Gly-Gly-X Repetitive Regions of Dragline Spider Silk” Int. J. Mol. Sci. 2016, 17, 2023.* [Invited Article, Special Issue on Silk-based Materials], IF = 3.257

Brown, J.E., Davidowski, S.K., Xu, D., Cebe, P., Onofrei, D., Holland, G.P., Kaplan, D.L. “Thermal and Structural Properties of Silk Biomaterials Plasticized by Glycerol” Biomacromolecules 2016, 17, 3911-3921, IF = 5.750

Finkelstein-Shapiro, D., Davidowski, S.K., Lee, P., Guo, C., Holland, G.P. Rajh, T., Gray, K.A., Yarger, J.L., Calatayud, M. “Direct Evidence of Chelated Geometry of Catechol on TiO₂ by a Combined Solid-state NMR and DFT Study” J. Phys. Chem. C. 2016, 120, 23625-23630.* IF = 4.509

Koyanagi, T., Leriche, G., Yep, A., Onofrei, D., Holland, G.P., Mayer, M., Yang, J. “Effect of Headgroups on Small-Ion Permeability Across Archae-Inspired Tetraether Lipid Membranes” Chemistry-A European Journal. 2016, 22, 8074-8077. IF = 5.771

Davidowski, S. and Holland, G.P. “Solid-state NMR Characterization of Mixed Phosphonic Acid Ligand Binding and Organization on Silica Nanoparticles” Langmuir 2016, 32, 3252-3261.* IF = 3.993

Koyanagi, T., Leriche, G., Onofrei, D., Holland, G.P., Mayer, M., Yang, J. “Cyclohexane Rings Reduce Permeability to Small Ions in Archaea-inspired Tetraether Lipids” Angew. Chem. Int. Ed. Engl. 2016, 55, 1890-1893. IF = 11.709

2015

Guo, C. and Holland, G.P. “Alanine Adsorption and Thermal Condensation at the Interface of Fumed Silica Nanoparticles: A Solid-state NMR Investigation” J. Phys. Chem. C. 2015, 119, 25663-25672.* IF = 4.509

Guzman, C.X., Calderon, R.M.K., Li, Z., Yamazaki, S., Peurifoy, S.R., Guo, C., Davidowski, S.K., Mazza, M.M.A., Han, Xu, Holland, G.P., Scott, A.M., Braunschweig, A.B. “Extended Charge Carrier Lifetimes in Hierarchical Donor-Acceptor Supramolecular Polymer Films” J. Phys. Chem. C. 2015, 119, 19584-19589. IF = 4.509

Xu, D., Guo, C., Holland, G.P. “Probing the Impact of Acidification on Spider Silk Assembly Kinetics” Biomacromolecules 2015, 16, 2072-2079.* IF = 5.750

Shi, X., Holland, G.P., Yarger, J.L. “Molecular Dynamics of Spider Dragline Silk Fiber Investigated by ²H MAS NMR” Biomacromolecules 2015, 16, 852-859.* IF = 5.750

2014

Guo, C. and Holland, G.P. “Investigating Lysine Adsorption on Fumed Silica Nanoparticles” J. Phys. Chem. C. 2014, 118, 25792-25801.* IF = 4.509

Tarakeshwar, P., Palma, J.L., Holland, G.P., Fromme, P., Yarger, J.L., Mujica, V. “Probing the Nature of Charge Transfer at Nano-Bio Interfaces: Peptides on Metal Oxide Nanoparticles” J. Phys. Chem. Lett. 2014, 5, 3555-3559. IF = 8.539

Xu, D., Yarger, J.L., Holland, G.P. “Exploring the Backbone Dynamics of Native Spider Silk Proteins in Black Widow Silk Glands with Solution NMR Spectroscopy” Polymer, 2014, 55, 3879-3885.* IF = 3.775

Addison, J.B., Popp, T.M.O., Weber, W.S., Edgerly, J.S., Holland, G.P., Yarger, J.L. “Structural Characterization of Nanofiber Silk Produced by Embiopterans (webspinners)” RSC Advances, 2014, 4, 41301-41313. IF = 3.289

Shi, X., Yarger, J.L., Holland, G.P. “Elucidating Proline Dynamics in Spider Dragline Silk Fiber Using ²H-¹³C HETCOR MAS NMR” Chem. Commun. 2014, 50, 4856-4859.* IF = 6.567

Addison, J.B., Weber, W.S., Mou, Q., Ashton, N.N., Stewart, R.J., Holland, G.P., Yarger, J.L. “Reversible Assembly of b-sheet Nanocrystals within Caddisfly Silk” Biomacromolecules 2014, 15, 1269-1275. IF = 5.750

2013

Asakura, T., Suzuki, Y., Nakazawa, Y., Holland, G.P., Yarger, J.L. “Elucidating Silk Structure Using Solid-state NMR” Soft Matter 2013, 9, 11440-11450. IF = 3.798

Jenkins, J.E., Sampath, S., Butler, E., Kim, J., Henning, R.W., Holland, G.P., Yarger, J.L. “Characterizing the Secondary Protein Structure of Black Widow Dragline Silk Using Solid-state NMR and X-ray Diffraction” Biomacromolecules 2013, 14, 3472-3483.* IF = 5.750

Fritzsching, K.J., Kim, J., Holland, G.P. “Probing Lipid-cholesterol Interactions in DOPC/eSM/Chol and DOPC/DPPC/Chol Model Lipid Rafts with DSC and ¹³C Solid-state NMR” Biochim. Biophys. Acta 2013, 1828, 1889-1898.* IF = 3.687

Holland, G.P., Mou, Q., Yarger, J.L. “Determining Hydrogen-bond Interactions in Spider Silk with ¹H-¹³C HETCOR Fast MAS Solid-state NMR and DFT Proton Chemical Shift Calculations” Chem. Commun. 2013, 49, 6680-6682.* IF = 6.567

Shi, X., Holland, G.P., Yarger, J.L. “Amino Acid Analysis of Spider Dragline Silk Using ¹H NMR Spectroscopy” Anal. Biochem. 2013, 440, 150-157.* IF = 2.219

Shi, X., Yarger, J.L., Holland, G.P. “Probing Site-specific ¹³C/¹⁵N-isotope Enrichment of Spider Silk with Liquid-state NMR Spectroscopy” Anal. Bioanal. Chem. 2013, 405, 3997-4008.* IF = 3.578

Addison, J.B., Ashton, N.N., Weber, W.S., Stewart, R.J., Holland, G.P., Yarger, J.L. “b-sheet Nanocrystalline Domains Formed from Phosphorylated Serine-rich Motifs in Caddisfly Larval Silk: A Solid-state NMR and XRD Study” Biomacromolecules 2013, 14, 1140-1148. IF = 5.750

Asakura, T., Suzuki, Y., Nakazawa, Y., Yazawa, K., Holland, G.P., Yarger, J.L. “Silk Structure Studied with Nuclear Magnetic Resonance” Prog. Nucl. Mag. Res. Sp. 2013, 69, 23-68. IF = 7.237

Shi, X., Yarger, J.L., Holland, G.P., “²H-¹³C HETCOR MAS NMR for Indirect Detection of ²H Quadrupole Patterns and Spin-lattice Relaxation Rates” J. Magn. Reson. 2013, 226, 1-12.* IF = 2.889

Pizzarello, S., Davidowski, S.K., Holland, G.P., Williams, L.B. “Processing of Meteoritic Organic Materials as a Possible Analog of Early Molecular Evolution in Planetary Environments” Proc. Natl. Acad. Sci. U.S.A 2013, 110, 15614-15619. [Featured on Spectroscopy Now] IF = 9.423

2012

An, B., Jenkins, J.E., Sampath, S., Holland, G.P., Hinman, M., Yarger, J.L., Lewis, R.V. “Reproducing Natural Spider Silk’s Copolymer Behavior in Synthetic Silk Mimics” Biomacromolecules 2012, 13, 3938-3948. IF = 5.750

Blanchard, J.W., Groy, T.L., Yarger, J.L., Holland, G.P. “Investigating Hydrogen-bonded Phosphonic Acids with Proton Ultrafast MAS NMR and DFT Calculations” J. Phys. Chem. C. 2012, 116, 18824-18830.* IF = 4.509

Jenkins, J.E., Holland, G.P., Yarger, J.L. “High Resolution Magic Angle Spinning NMR Investigation of Silk Protein Structure within Major Ampullate Glands of Orb Weaving Spiders” Soft Matter 2012, 8, 1947-1954.* IF = 3.798

Teule, F., Addison, B., Copper, A.R., Ayon, J., Henning, R.W., Benmore, C.J., Holland, G.P., Yarger, J.L., Lewis, R.V. “Combining Flagelliform and Dragline Spider Silk Motifs to Produce Tunable Synthetic Biopolymer Fibers” Biopolymers 2012, 97, 418-431. IF = 2.248

2011

Spektor, K., Nylen, J., Navrotsky, A., Hervig, R.L., Leinenweber, K., Holland, G.P., Haussermann, U. “Ultrahydrous Stishovite from High-pressure Hydrothermal Treatment of SiO₂” Proc. Natl. Acad. Sci. U.S.A. 2011, 108, 20918-20922. IF = 9.423

Bo, A., Hinman, M.B., Holland, G.P., Yarger, J.L., Lewis, R.V. “Inducing b-sheets Formation in Synthetic Spider Silks by Aqueous Post-spin Stretching” Biomacromolecules 2011, 12, 2375-2381. IF = 5.750

Blanchard, J.W., Belieres, J.-P., Alam, T.M., Yarger, J.L., Holland, G.P. “NMR Determination of the Diffusion Mechanisms in Triethylamine-based Protic Ionic Liquids” J. Phys. Chem. Lett. 2011, 2, 1077-1081.* IF = 8.539

Pizzarello, S., Williams, L.B., Lehman, J., Holland, G.P., Yarger, J.L. “Abundant Ammonia in Primitive Asteroids and the Case for a Possible Exobiology” Proc. Natl. Acad. Sci. U.S.A. 2011, 108, 4303-4306. IF = 9.423